Bioluminescent & Fluorescent Imaging Core

The Bioluminescent and Fluorescent Imaging Core offers in vitro and in vivo biophotonic imaging services using bioluminescence and/or fluorescent molecules as reporters of gene or protein expression.

Technology Description

Noninvasive in vivo bioluminescent imaging permits the detection of light-producing biological reactions in living animals and illuminates biological processes in vivo. The technology provides a “window” into the organism, and makes possible the tracking of biological activity in real time, at the molecular level.

The technology uses luciferases and other fluorescent proteins that make some insects, jellyfish and bacteria glow. The luciferase gene is incorporated into cells, microorganisms and animals. By analyzing the light emission, it is possible to monitor cellular or genetic activity and use the results to track gene expression, the spread of a disease, or the effect of a new drug candidate in vivo at the single photon level. The technology has applications in multiple research areas including osteomyelitis, oncology, inflammation and metabolism.

Instrumentation

The core facility houses a Caliper Life Sciences IVIS 200 Imaging System, a highly sensitive CCD camera optimized for biophotonic imaging. The system acquires a photographic image of mice overlayed with a bioluminescent or fluorescent image. The intensity of the bioluminescent or fluorescent image is displayed in a pseudocolor scale in which blue regions represent lower photon emission and red regions represent greatest photon emission. 

Contact Information

Bioluminescent & Fluorescent Imaging Core
Winthrop P. Rockefeller Cancer Institute, 9th floor
4301 West Markham Street
Mail Slot 644
Little Rock, AR 72205

Larry Suva, Ph.D., Director
Department of Orthopaedic Surgery
UAMS College of Medicine
ljsuva@uams.edu
501-526-6110